PCR Equipment and Supplies
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Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)
Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | Medium |
| Fidelity (vs. Taq) | 1X |
| Format | Tube |
| Size (Final Product) | 5 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start PCR Master Mix |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Overhang | 3'-A |
| Concentration | 2X |
| Starting Material | DNA |
| For Use With (Application) | Hot-start PCR |
Thermo Scientific™ Phusion Blood Direct PCR Kit
Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Phusion Blood II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Reaction Format | Separate Components |
| Product Type | Direct PCR Kit |
| Sample Type | Blood |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2 U/μL |
| Starting Material | DNA |
| PCR Method | Direct PCR |
Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Ambion Single Cell-to-CT™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Sample Type | Mammalian Cells |
| Reaction Speed | 7 min. |
| Purification Time | 7 min. |
| Starting Material | 1-10 Cells |
| For Use With (Application) | RT-PCR |
| PCR Method | 2-step RT-qPCR |
| Product Line | Ambion, Cells-to-CT |
| Detection Method | TaqMan |
Invitrogen™ Taq DNA Polymerase, recombinant
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
| PCR Method | qPCR,Standard PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | Taq DNA Polymerase |
| Format | Stand-alone enzyme |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | No |
| Starting Material | DNA |
Applied Biosystems™ SNaPshot™ Multiplex Kit
The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Product Type | Multiplex Kit |
|---|---|
| For Use With (Equipment) | 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers |
| Sample Type | DNA |
| Technique | SNP Genotyping (Fragment Analysis-Based), Primer Extension |
| Product Line | SNaPshot |
| Detection Method | Primer-probe |
Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Control Primer-Probe Set(s) |
| Gene Symbol | 18S |
| Amplicon Size | 187 |
| Concentration | 20X |
| For Use With (Application) | Gene Expression |
| PCR Method | 2-step RT-qPCR |
| Detection Method | Primer-probe |
| Accession Number | X03205.1 |
| Target | 18S rRNA |
| Format | Tube |
| Label or Dye | FAM |
| Species | Eukaryotic, Rat, Mouse, Human |
| Product Line | FAM, TaqMan |
Applied Biosystems™ Mouse GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited)
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Mouse GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Cells-to-CT Bulk RT Reagents
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan™ Gene Expression Cells-to-CT™ Kit (20X RT enzyme mix and 20X RT buffer).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Phire Reaction Buffers
The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents or Green version containing a density reagent and two tracking dyes for direct loading of PCR products on gel.
| Quantity | 4 x 1.5 mL |
|---|---|
| Concentration | 5X |
Invitrogen™ Buffer Kit, RNase-free
The Invitrogen Buffer Kit provides high quality, RNase free buffers for critical RNA analysis procedures.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ MeltDoctor™ HRM Positive Control Kit
Provides nucleic acid controls to demonstrate and troubleshoot High Resolution Melt (HRM) analysis
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit
ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Target | DNA from Enzymatic Reactions (e.g. PCR) |
| Content And Storage | The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. |
| Format | Kit |
| Product Type | PCR Clean-Up Kit |
| Sample Type | PCR Products |
| Isolation Technology | ChargeSwitch™ Chemistry |
| Run Time | 5 min |
| For Use With (Application) | Next-Generation Sequencing (NGS),Cloning,Standard PCR |
| High-throughput Compatibility | High-throughput Compatible |
| Product Line | ChargeSwitch |
Applied Biosystems™ GeneAmp™ dNTP Blend (100 mM)
GeneAmp dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. Each vial contains a blend of 100 mM solution of dNTP (25 mM each of dATP, dCTP, dGTP and dTTP).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Form | Liquid |
| Concentration | 100 mM |
| For Use With (Application) | Standard PCR |
| Product Line | GeneAmp |
Invitrogen™ Cells-to-cDNA™ II Kit
The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature. |
| Reverse Transcriptase | M-MLV |
| Size (Final Product) | 7 kb or less |
| Final Product Type | cDNA |
| Sample Type | Mammalian Cells |
| Purification Time | 2 hr. |
| Optimal Reaction Temperature | 42°C |
| For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
| Product Line | Ambion, Cells-to-cDNA |
Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit
The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Format | Kit |
| Reverse Transcriptase | MultiScribe |
| Size (Final Product) | Up to 7 kb |
| Reaction Format | Premixed Components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 30 to 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Yes |
| Optimal Reaction Temperature | 37°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
| For Use With (Application) | Real Time PCR (qPCR),RT-PCR |