PCR Equipment and Supplies
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Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temp or Wet Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Hot-start PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | AmpliTaq Gold 360 DNA Polymerase |
| Format | Tube |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Invitrogen™ Platinum™ SuperFi™ PCR Master Mix
Invitrogen Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix
The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ SuperScript™ IV RT Reaction Buffer
SuperScript IV RT Reaction Buffer is included with, and designed for use with, SuperScript IV Reverse Transcriptase.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ SuperScript™ III Reverse Transcriptase
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Reaction Speed | 30 to 50 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ PCRX Enhancer System
PCRx Enhancer System is used in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR
SuperScript III First-Strand Synthesis SuperMix for qRT-PCR provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Format | Super Mix |
| Reverse Transcriptase | SuperScript III |
| Reaction Format | Master Mix |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
| For Use With (Application) | Real Time PCR (qPCR) |
| Detection Method | qPCR |
Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | First-Strand cDNA |
| For Use With (Application) | Real Time PCR (qPCR) |
| Detection Method | qPCR |
| Format | Kit |
| Size (Final Product) | Up to 10 kb |
| Reaction Format | Master Mix |
| Reaction Speed | 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 42°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ Platinum™ SuperFi™ DNA Polymerase
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | PCR Amplified cDNA |
| PCR Method | 1-step RT-PCR |
| Detection Method | Gel Electrophoresis |
| Polymerase | Platinum Taq High Fidelity |
| Format | One-Step RT-PCR System |
| Size (Final Product) | Up to 10 kb |
| Reaction Format | Premixed Components |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Hot Start | Built-In Hot Start |
| Optimal Reaction Temperature | 50°C |
| Technique | 1-Step RT-PCR |
| Starting Material | RNA |
Invitrogen™ 5-(3-Aminoallyl)-dUTP (50 mM)
When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Poly(A) Tailing Kit
For the polyadenylation of in vitro transcribed RNA to enhance translation initiation efficiency.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6), Alexa Fluor™ Plus 555
Rabbit Recombinant Monoclonal Antibody
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Content And Storage | 4°C, store in dark, DO NOT FREEZE! |
|---|---|
| Target Species | Human |
| Host Species | Rabbit |
| Conjugate | Alexa Fluor Plus 555 |
| Applications | Flow Cytometry,Immunocytochemistry |
| Form | Liquid |
| Gene Accession No. | Q01860 |
| Isotype | IgG |
| Concentration | 1.0 mg/mL |
| Antigen | OCT4 |
| Gene Symbols | POU5F1 |
| Regulatory Status | RUO |
| Purification Method | Protein A |
| Gene Alias | 2-Oct; 3-Oct; 4-Oct; cb197; chunp6868; DADB-104B20.2; etID49452.21; gp9; gp-9; HGNC:9221; homeobox protein; lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; MGC22487; NF-A3; Oct2; Oct-2; Oct2a; Oct2b; OCT3; Oct-3; Oct3/4; Oct-3/4; OCT4; Oct-4; Octamer-binding protein 2; octamer-binding protein 3; Octamer-binding protein 4; octamer-binding transcription factor 2; Octamer-binding transcription factor 3; OTF2; OTF-2; OTF3; Otf-3; Otf3g; Otf3-rs7; Otf4; Otf-4; OTTHUMP00000221150; OTTHUMP00000221151; POU class 2 homeobox 2; POU class 5 homeobox 1; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain gene 2; POU domain protein 2; POU domain transcription factor OCT4; POU domain, class 2, transcription factor 2; POU domain, class 5, transcription factor 1; POU domain, class 5, transcription factor 3; pou2; pou-2; POU2F2; Pou5f1; pou5f1 protein; pou5f3; POU-type homeodomain-containing DNA-binding protein; spg; spg/pou2; spiel-ohne-grenzen; spiel-ohne-grenzen/pou2; wu:fd18d06 |
| Gene | POU5F1 |
| Product Type | Antibody |
| Gene ID (Entrez) | 5460 |
| Formulation | Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8 |
| Immunogen | Protein corresponding to Human POU5F1 (aa 1-360). |
| Classification | Recombinant Monoclonal |
| Primary or Secondary | Primary |
| Clone | 3H8L6 |
Thermo Scientific™ Exo-Resistant Random Primer
Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions.
Invitrogen™ PureLink™ PCR Micro Kit
PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Separation Range | 100 bp to 12 kb |
| Content And Storage | Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature. |
| Starting Material Volume | 125 μL |
| Isolation Technology | Silica Spin Column |
| Elution Volume Max | 20 μL |
| For Use With (Application) | Cloning,Standard PCR |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Target | DNA from Enzymatic Reactions (e.g. PCR) |
| Format | Spin Column |
| Product Type | PCR Micro Kit |
| Run Time | 10 min. |
| Elution Volume Min | 5 μL |
| Product Line | PureLink |